Pustaka Ilmiah Universitas Padjadjaran

Abstrak

A Study To Predict Anti-Inflammatory Activity Of Eugenol, Myristicin, And Limonene Of Cinnamomum Sintoc

Objective: In this work we predicted anti-inflammatory activity of volatile oil of C sintoc L. Methods: Molecular docking was performed to predict the binding modes of eugenol, myristicin, and limonene chemical constituents of C. sintoc L with COX enzymes, using Auto Dock 4.2. COX enzymes were obtained from Protein Data Bank (PDB); COX-1 (PDB code: 2AYL) and COX-2 (PDB code: 3PGH). Flurbiprofen and celecoxib were used as standards. Further assay was carried out on lipopolysaccharide (LPS)-induced fibroblast cells reacted with 800; 400; 200; 100; 50; 25 and 12.5 ul of C sintoc L. bark essential oils. The absorbance of the product was measured using microplate reader at 450 nm. Acetosal was used as the standard drug. Results: Eugenol and myristicin could be categorized as non-selective inhibitors of COX-2, while limonene is categorized as preferential COX-2 inhibitor. The essential oils of C sintoc L bark reduced PGE2 production on LPS-induced fibroblast cells. The inhibitory activity of C. sintoc L was weaker than acetosal. Conclusion: Bioactive compounds in essential oil of C sintoc L bark show inhibition on PGE2 production on LPS-induced human fibroblast cells, and could be categorized as COX inhibitors.