Abstrak 
The Anti-hypertensive Effect Of Isolate From Active Fraction Of Roselle (Hibiscus Sabdariffa L.) On White Male Rats
Yasmiwar Susilawati, Sri Adi Sumiwi, Eneng Nuraisah
Universitas Padjadjaran, Faculty of Pharmacy, Universitas Padjadjaran, Proceeding The International Seminar and Expo on Jamu 2010 (ISEJ 2010) “Herbal Medicines : Indigeneous, Molecular Aspects, and Clinical Application” Bandung, Indonesia, 3-6 November 2010, ISBN 978-602-96121-1-0
Bahasa Inggris
Universitas Padjadjaran, Faculty of Pharmacy, Universitas Padjadjaran, Proceeding The International Seminar and Expo on Jamu 2010 (ISEJ 2010) “Herbal Medicines : Indigeneous, Molecular Aspects, and Clinical Application” Bandung, Indonesia, 3-6 November 2010, ISBN 978-602-96121-1-0
Anti- hypertensive, Hibiscus sabdarifffa, Roselle calyces
Roselle (Hibiscus sabdariffa) is a medicinal plant that have long been used for treating hypertension. The previous study had been known that the ethyl acetate fraction of roselle calyces showed the strongest antihypertensive effect if it was compared with n-hexane fraction and ethanol fraction. This research has purpose to know the antihypertensive effect of isolate from ethyl acetate fraction of roselle calyces in white male rats. The isolation process was started with extraction of crude material continuously by Soxhlet apparatus using n-hexane, ethyl acetate, and ethanol. The ethyl acetate extract was fractionated by vacuum liquid chromatography (VLC) and column chromatography, followed by isolation using preparative thin layer chromatography yielding HS-II-B1, HS-II-B2 and HS-II-B3. The experiment of antihypertensive effect of each isolate was done using indirect method at dose of 30, 60 and 20 µg/kg of body weigt successively. Captopril at dose of 4.5 mg/kg of body weight was used as a positive control. Hypertension was induced by administrating of adrenalin at a dose of 1.2 µg/kg of body weight to the testing animal. The oral administration of each isolate showed the activity of reduction in systolic blood pressure successively 27.20 %; 18.76 and 16.05 % (ANAVA p > 0,05), whereas the activity of reduction in diastolic blood pressure at the same dose successively 25.09 %; 21.22 % and 8.93 % (ANAVA p < 0,01), both was compared with negative control. The antihypertensive activity of each isolate could be arranged based on the average of percentage of reduction in systolic and diastolic blood pressure, successively was Hs-II-B1, Hs-II-B2 dan HS-II-B3.