Abstrak RSS

The Role of L. plantarum as an Immunomodulator Secretion of Transforming Growth Factor-ß1, Transforming Growth Factor-ß3, and Interferon-a Macrophages and Dermal Fibroblasts

The Role of L. plantarum as an Immunomodulator Secretion of Transforming Growth Factor-ß1, Transforming Growth Factor-ß3, and Interferon-a Macrophages and Dermal Fibroblasts
Rita Shintawati, Sunarjati Sudigdoadi, Tita Husnitawati Madjid, Endang Sutedja
Universitas Padjadjaran, Asian Journal of Applied Sciences (ISSN: 2321 – 0893) Volume 05 – Issue 02, April 2017
Bahasa Inggris
Universitas Padjadjaran, Asian Journal of Applied Sciences (ISSN: 2321 – 0893) Volume 05 – Issue 02, April 2017
, , ,

This research aims to see the effect of L.plantarum in modulating the secretion of Transforming Growth Factor-TGFß1, TGFß3 macrophages and fibroblasts, Interferon-IFNa macrophages, and to analyze the possibility of L.plantarum potency in supporting the process of scarless wound healing. The culture of peritoneal macrophages was treated with L.plantarum for 24 hours, while another macrophage was S.aureus stimulated for 6 hours before treatment of L.plantarum for 24 hours. The formed supernatant was separated and centrifuged to serve as a treatment on the culture of rat dermal fibroblasts for 24 hours. The supernatant was then separated and centrifuged; its cytokine level was measured with enzyme-linked immunosorbent assay-ELISA. Treatment of L.plantarum with medium and high doses increased the secretion of IFNa macrophages compared with the control; all L.plantarum doses can stimulate the secretion of TGFß1 fibroblast and TGFß3 macrophage significantly, but it does not affect the secretion of TGFß1 macrophages. It can be concluded that L.plantarum increased the secretion of IFNa macrophages higher than the treatment preceded by S.aureus stimulation. The secretion of TGFß1 fibroblasts and TGFß3 fibroblasts also increased, but it was not as high as L.plantarum treatment stimulated by S.aureus. Therefore, the application of L.plantarum to support the process of wound healing, prophylactic of the excessive scar and fibrosis can be researched further.

Download: .Full Papers