Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Correlation of Total Antioxidant Capacity Measured by Ferric Reducing Ability of Plasma (FRAP) Assay with the Severity of Preeclampsia ( Hubungan Kapasitas Antioksidan Total yang diukur dengan Metode Ferric Reducing Ability of Plasma (FRAP) dengan Derajat Beratnya Penyakit Preeklampsia)

Tujuan: Untuk menganalisis hubungan antara kapasitas antioksidan Total yang diukur dengan metode FRAP dengan derajat beratnya penyakit preeklampsia. Metode: Penelitian dilakukan secara potong silang dengan membandingkan KAT dengan metode FRAP pada empat kelompok penelitian yaitu kehamilan normal, preeklampsia ringan, preeklampsia berat serta eklampsia. Setiap kelompok berjumlah 15 orang yang memenuhi kriteria inklusi dan datang berobat ke RSUP Dr. Hasan Sadikin Bandung Serta rumah sakit jejaringnya, berlangsung dari bulan Agustus – September 2011. Sampel darah dipergunakan Sebanyak 3 ml lalu diperiksakan di laboratorium PRODIA Jakarta. Hasil: Tidak terdapat perbedaan bermakna (p>0,05) pada karakteristik subjek penelitian dari segi jumlah paritas dan usia kehamilan. Kadar FRAP pada keempat kelompok penelitian berdasarkan uji ANOVA menunjukkan perbedaan sangat bermakna (p<0,01). Kadar FRAP tertinggi Ditemukan pada kelompok eklampsia dengan rerata 1441,1 ±315,8, diikuti preeklampsia berat 1118,8± 118,3, preeklampsia ringan 902,4±102,5 serta kehamilan normal 769,3 ±117,1. Berdasarkan uji korelasi Rank Spearman terdapat korelasi positif yang bermakna (p<0,05) dengan rasio sebesar 0,880 antara kadar FRAP dengan derajat berat penyakit preeklampsia. Berdasarkan uji prevalensi rasio dengan CI 95% didapatkan risiko sebesar 2,17 kali pada kadar FRAP = 769,3 untuk terjadi Preeklampsia ringan serta risiko sebesar 2,5 kali untuk terjadi preeklampsia berat atau eklampsia. Kesimpulan: Didapatkan korelasi positif dengan kekuatan sangat kuat antara kadar FRAP dengan derajat berat penyakit preeklampsia. Objective: To analyze correlation between total antioxidant capacity Measured by Ferric Reducing Ability of Plasma (FRAP) assay With severity of preeclampsia. Method: The cross sectional method was used in this study to compare TAC of four different groups of study, consists of normal pregnancy, mild preeclampsia, severe preeclampsia and eclampsia. The study consisted of 15 women in each group. All of the subjects met the inclusion criteria and were admitted to Dr. Hasan Sadikin Hospital and it’s district hospital. The study was conducted from August until September 2011. 3 ml blood samples were taken and were measured by FRAP assay in the laboratory PRODIA Jakarta. Result: There was no significant difference (p<0.05) on subject’s characteristics based on number of parity and gestational age among the groups of study. The comparison of TAC measured by FRAP Assay based on ANOVA was significant difference (p<0.01). The Highest mean FRAP assay result appeared in eclampsia which was 1441.1 ±315.8, while in severe preeclampsia 1118.8±118.3, mild preeclampsia 902.4 ±102.5 and in normal pregnancy 769.3± 117.1. There was significant (p<0.05) positive correlation (ratio 0.880) between TAC measured by FRAP assay with severity of preeclampsia. Based on prevalence ratio with CI 95% subjects with FRAP level = 769.3 had about 2.17 times higher risk to develop mild preeclampsia and subjects with FRAP level = 769.3 had about 2.5 times higher risk to develop severe preeclampsia or eclampsia. Conclusion: There was a very strong positive correlation between TAC measured by FRAP assay with the severity of preeclampsia.