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The Intracellular Cryoprotectant Effects In Preserving Goramy Spermatozoa After Two Days Sub-zero Freezing
The Intracellular Cryoprotectant Effects In Preserving Goramy Spermatozoa After Two Days Sub-zero Freezing
Abinawanto, Nisa Fitrianingrum, Retno Lestari, Agung Sudaryono, Rita Rostika, Yushinta Fujaya
Universitas Padjadjaran, Aquacultura Indonesiana (2015) 16 (1) : 16-21, ISSN 2477-6939, www.aquasiana.org
Bahasa Inggris
Universitas Padjadjaran, Aquacultura Indonesiana (2015) 16 (1) : 16-21, ISSN 2477-6939, www.aquasiana.org
Glycerol, Osphronemus goramy, Spermatozoa motility, Sub-zero freezing, Viability and Abnormality
Abinawanto, Nisa Fitrianingrum, Retno Lestari, Agung Sudaryono, Rita Rostika, Yushinta Fujaya
Universitas Padjadjaran, Aquacultura Indonesiana (2015) 16 (1) : 16-21, ISSN 2477-6939, www.aquasiana.org
Bahasa Inggris
Universitas Padjadjaran, Aquacultura Indonesiana (2015) 16 (1) : 16-21, ISSN 2477-6939, www.aquasiana.org
Glycerol, Osphronemus goramy, Spermatozoa motility, Sub-zero freezing, Viability and Abnormality
The spermatozoa quality of goramy after 2 d sub-zero freezing was examined. The quality of spermatozoa examined included motilit y, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4 °C for 45 min, and were freezed at -34°C for 2 d. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.
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