Abstrak 
Comparison of Different Methods for Purification of Gold Nanoparticles– Polyamidoamine (PAMAM) Generation 4 Dendrimer
Narsa Angga Cipta, Pujiyanto Anung, Gunawan Adang H., Yusup Iyus Maolana, Listyowati Indrarini, Fauzia Retna Putri, Bratadiredja Marline Abdassah, Mutalib Abdul, Soetikno Ristaniah D., Soedjanaatmadja R. Ukun M.S.
Universitas Padjadjaran, Research Journal of Chemistry and Environment Vol. 22(Special Issue II) August (2018), E-ISSN: 2278 – 4527, PRINT-ISSN No. 0972-0626
Bahasa Inggris
Universitas Padjadjaran, Research Journal of Chemistry and Environment Vol. 22(Special Issue II) August (2018), E-ISSN: 2278 – 4527, PRINT-ISSN No. 0972-0626
Contrast agents, CT-scan, dendrimer, Gold nanoparticles, purification
Gold nanoparticles (AuNP) are currently being widely applied in cancer diagnosis as a CT Scan contrast agent (imaging) as well asin therapy (theragnostic agents). The polyamidoamine (PAMAM) G4 dendrimer has also been applied as a stabilizer agent to control the size of gold nanoparticles. In the production of AuNP-PAMAM G4 dendrimer, a solvent and reducing agent were used. Therefore, it is necessary to separate and purify the synthesis result of AuNP-PAMAM G4 dendrimer from the undesired compounds. Separation and purification were carried out by two different methods, namely, size exclusion column chromatography Sephadex G25® (SE), and membrane ultrafiltration (MU). The percent yield of AuNP that has been synthesized was calculated by using Inductively Couple Plasma Atomic Emission Spectroscopy (ICP-OES) and then characterized by using UV-Vis Spectroscopy and Transmission Electron Microscope (TEM). The percent yield of AuNP, which was carried out by MU, was 93.75%. This result was better compared to using SE (81.18%). The characteristics results from the UV-Vis spectrum demonstrated similar spectra between separation and purification methods using both MU and SE. The characteristics results from TEM also showed similar morphological results.