Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Combined Effect Of Nacl And Iba On Ms Media On Growth Of Rice Mutant Strain In Vitro

The Influence of NaCl and IBA at the MS media for the growth of rice mutant in vitro was conducted in the tissue culture laboratory and green house plant’s breeding group (PATIR BATAN). The aims of this research was to know the concentrate variation of NaCl, IBA, and MS media combination for growing percentage, height and root length in planlet Diah Suci variety and three rice mutant lines. The experiment was arranged in Completely Randomized Design, with three factors, fourty eight treatments and five replications. The first factor was four concentration of NaCl (0%, 0.5%, 1% and 1.5%). The second factor was three concentration of IBA (0%, 2.5% and 5%). The third factor was Diah Suci variety as plant control and three rice mutant lines (Obs 1700, Obs 1701, and Obs 1704. The result of four weeks observation was show that the added of NaCl up to 1.5%, so the growth percentage, height and root length of plantlet would be to descend. The concentration of IBA until 5% influenced to the growth percentage, height and root length of planlet. The combination of three parameter refered to Obs 1704 better than control and the others (Obs 1700 and Obs 1701) with the growth percentage was 80%, planlet height 19.84 cm and root length 5.62 cm in combination of NaCl 1% and IBA 5% on four weeks observation.