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Hydrolysis Of Sugarcane Bagasse (Saccharum Officinarum L.) By Acid-enzyme Combinations And Further Fermentation Of The Hydrolysate By Pichia Stipitis, Saccharomyces Cerevisiae, And Zymomonas Mobilis

Hydrolysis Of Sugarcane Bagasse (Saccharum Officinarum L.) By Acid-enzyme Combinations And Further Fermentation Of The Hydrolysate By Pichia Stipitis, Saccharomyces Cerevisiae, And Zymomonas Mobilis
Ratu Safitri, In-in Hanidah, Toto Subroto
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Sugarcane bagasse (Saccharum officinarum L.) is a readily available waste product of cane-sugar processing. The major components of bagasse are cellulose and hemicellulose. The objective of the research was to produce bio-ethanol using bagasse as raw materials, involving optimization of pretreatments, sulphuric acid hydrolysis, enzyme hydrolysis using respectively cellulose and hemicellulase; and fermentation of the hydrolysate by three types of microorganisms, P. stipitis CBS 5773, or S. cerevisiae D1/P3GI, or Z. mobilis 0056 FNCC respectively. The experiment employed descriptive analyses in triplicates. The results were as follows: Pretreatments of 1 : 10 (w/v) sugarcane bagasse of 30 mesh particle size required a thermal process of 30 minutes at 120oC; the sulphuric acid hydrolysis was best by using a 2% (w/w) sulphuric acid solution and heating for 60 minutes at 120oC; the enzymatic hydrolysis was the best when using hemicellulase at a concentration of 0,001 g/g, followed by cellulase hydrolysis at a concentration of 0,083 ┬ÁL/g. This enzymatic hidrolyses was able to hydrolyze 63,52% of the bagasse lignocellulose, producing a hydrolysate containing 32,00 g/L reducing sugars, mainly glucose, xylose, and arabinose. Effectiveness of Z. mobilis 0056 FNCC was highest for producing bio-ethanol, 18,99 g/L within 3 hours. S. cerevisiae D1/P3GI produced 17,05 g/L bioethanol within 12 hours, and P. stipitis CBS 5773 13,03 g/L bio-ethanol within 24 hours respectively.

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