Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

The Profile Of Middle Digestive Tract (Midgut) Tissue Damage On Spodoptera Litura Fabricius Larvae Due To The Infection Of Helicoverpa Armigera Nuclear Polyhedrosis Virus (Hanpv)

The research to determine what can larva Spodoptera litura be used as an alternate host for Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) production has been conducted. The research has been done wthi laboratory experiments approach, using complete randomized design (10 treatments / infection time) and three repetitions, with concentration of PIB was 1.13 x 105 PIB/ml. The result was observed through creating histologic slide of midgut tissue damage of larva S. litura of fourth instar through Mallory-Azan staining method. The weight of larva was measured in observation time, and the present of Polyhedral Inclusion Body (PIB) in infected tissue was also monitored. Observation was conducted every 8 hours from the 1st until the 72nd hour after infection. Results showed that time treatments after the larva was infected by virus has significant impact (?< 0.05) on the middle section digestive tract (midgut) tissue damage and the presence of PIB. Damage has begun since the 8th hour after infection. It was characterized by the enlargement of columnar nucleus with PIB within columnar cell and the loss of peritrofic membrane. Observation at the 24th hour showed that almost all columnar cells had lisis, but at the 32nd hour the columnar cells have been regenerated. After the 64th up to 72nd hour after infection, all columnar cells lisis and PIB form into granule (polyhedral granules). Therefore larva S. litura can be used as an alternate host for HaNPV production.