Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Agronomic Trait Evaluation of Transgenic Rice Line With Db1 Transgene

Genetic improvement of rice crop resistance to brown planthopper is one of the main goals in rice breeding programs in Indonesia. To achieve this goal, Obi transgene (Mannose¬Binding Lectin Family Gene) driven by sucrose synthase-I promoter, which was isolated from Dioscorea batotos and proven to be effective against sup-sucking insect, had been succesfully transformed into rice genome cv.Toichung-65 by using Agrobacterium-mediated transformation and one homozygous line has been selected. However, plants derived from genetic transformation frequently show phenotypic abnormality, hence agronomic trait evaluation is required to clarify the occurrence of this phenomenon. The objective of this experiment was to obtain transgenic rice plants with normal phenotypes (cv. Taichung 65). Forty rice plants were used in this experiment, consist of 20 T3 generation of transgenic rice plants cv. Taichung-65 and 20 non-transgenic rice lines of cv. Taichung-65 as check. Data were analyzed by student’s t-test. Significant differences were found in heading dote, days to panicle exertion, plant height, wet weight of panicle, dry weight of panicle, grain number per panicle, grain weight per panicle, and grain filled per hill. These results suggest that agronomic trait differences in transgenic plants caused by somaclonal variation during in vitro culture and transformation events.