Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Effectiveness Of Internal Chemical Indicator In The Form Of Strips For Class IV And V Using Steam Sterilization

Infections through contaminated medical equipments are a serious threat to any patient’s safety and can jeopardise this treatment or may lead to new illness. Such risk exposures has always been treated seriously by hospitals, to ensure every medical procedures are well monitored to mitigate any probability of such risk exposure. Sterilization process is a compulsory procedure to ensure sterility of medical equipments where chemical indicators are broadly used to monitor sterilization process. The purpose of this study is to determine the development of internal chemical indicators in the form of strips of class IV and V in the steam sterilization in order to obtain good and quality products. Internal chemical indicator strips able to provide visual verification on products that have passed through the sterilization process, with colour change on the indicator. The stages in this research has also included microbial contamination test Laminar Air Flow (LAF), dosage formulations NaCl 0.9% infusion, application of internal chemical indicator strips in the moist heat sterilization process and evaluation of NaCI 0.9% infusion that includes sterility test. This study was carried comprising 7 variations of time with data that shows a positive reaction for both indicators with a change of colour after 121 °C of sterilization. Sterility test shows no microbial growth starting from the sterilization time of 10.5 minutes. This research has successfully concluded that internal chemical indicator strips class IV and V are effective on steam sterilization at 121 °C for 10.5 minutes and can be used to monitor the sterilization process.