Pustaka Ilmiah Universitas Padjadjaran

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Ekspresi Vascular Endothel Growth Factor-B (VEGF-B) Pada Adenokarsinoma Kolorektal

Adenokarsinoma kolorektal adalah tumor ganas yang menyusun struktur kalenjar dan berasal dari epitel kolorektal. Untuk pertumbuhan dan metastasis adenokarsinoma diperlukan proses anglogenesis Vascular endothelial growth factor-B (VEGF-B) adalah salah satu faktor penting dalam proses angiogenesis terutama pada adenokarsinoma Tujuan penelitian ini adalah untuk mengetahui korelasi ekspresl VEGF-B adenokarsinoma kolorektal dengan derajat keganasan. stadium (Duke), usia, jenis kelamin, dan tempat primer. Metoda penelitian adalah netrospektif terhadap 30 kasus adenokarsinoma kolorektal yang dilakukan perwarnaan imunohistokimia dengan menggunakan VEGF-B (Santa Cruz Biotechnology, Inc), Rantang usia pasien pada penelitian ini antara 19 sampai 80 tahun. terdiri dari 17 laki-laki dan 13 perempuan. Usia rata-rata adalah 47 tahun. Derajat keganasan adenokarsinoma kolorektal terdiri dari 20 kasus berdiferensiasi baik, 4 kasus berdiferenslasi sedang, dan 6 kasus berdiferensiasi buruk. Stadium tumor terdiri dari Duke A sebanyak 0 kasus, Duke B 17 kasus, Duke C 12 kasus, Duke D 1 kasus. Adenokarsinoma kolorektal terbanyak pada kolon reklosigmoid 17 kasus, Ekspresi VEGF-B terIlhat pada sitoplasma sel tumor dan mempunyai intensitas yang tersebar dari +1 (lemah), +2 (sedang), dan +3 (kuat). Untuk adenokarsinoma yang berdiferensiasi baik tercatat ekspresi VEGF-B +3 sebanyak 5 (25%) kasus, +2 sebanyak 13 (65%) kasus, dan +1 sebanyak 2 (10%) kasus. Derajat keganasan sedang +3 sebanyak 3 (75%) kasus dan +2 sebanyak 1 (25%) kasus. Derajat keganasan buruk sebanyak 6 (100%) kasus. Kesimpulan penelitian ini adalah ekspresi VEGF-B mempunyal intensitas yang tinggi terutama pada derajat keganasan buruk dibandingkan dengan derajat keganasan lainnya, tetapi tidak berhubungan dengan usia, Jenis kelamin, tempat primer. dan stadium adenokarsinoma kolorektal. Diharapkan hasil penelitian Ini dapat menjadi acuan untuk pemberian terapi Anti angiogenesis.

Colorectal adenocarcinoma is the glandular malignancy of colorectal epithelial layer. Angiogenesis process is needed for growth and metastasized of the adenocarcinoma and Vascular endothelial growth factor-B (VEGF-B) is one of the important factor in the angiogenesis process. This study was conducted to determine the correlation of vascular endothelial growth factor-B (VEGF-B) expression in colorectal adenocarcinoma with tumor grade, Duke’s stadium, age, sex, and primary location of the malignancy. This retrospective study collected 30 samples of colorectal adenocarcinoma and stained with immunohistochemistry method used VEGF-B (Santa Cruz Biotechnology, Inc). The age of the patient’s were 19-80 years-old, 17 were women and 13 were men. Mean age was 47 years old. The grade of colorectal adenocarcinoma were well differentiated in 20 cases, moderately differentiated in 4 cases, and six cases were poorly differentiated. None of the cases was Duke’s A, 17 cases were Duke’s B, twelve cases were Duke’s C, and only one case was Duke’s D. Mostly, the primary location of the malignancy were in rectosigmoid (17 cases). The expression of the V~GF-B was in the cytoplasm and the intensity range was +1 (weak), +2 (moderate) and +3 (strong). Well differentiated adenocarcinoma with strong expression of VEGF-B were detected in five cases (25%), moderate expression were 13 cases (65%), and weakly expression were 2 cases (10%). Moderately differentiated adenocarcinoma with VEGF-B strong expression were in 3 cases (75%), and moderate expression was one case (25%). Poorly differentiated adenocarcinoma with strong expression of VEGF-B were found in all six cases (100%). The conclusion revealed that the intensity of VEGF-B immunoexpression is higher in poorly differentiated adenocarcinoma colorectal than others grade, but do not correlate with age, sex, primary location of malignancy, and Duke’s stadium of the tumor. The result of this study hopefully could encourage anti-angiogenic therapy in such tumor