Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Design Of Hydroquinone Indicator Strip Based On Polymethylmethacrylate (PMMA) For Identification Of Hydroquinone In Whitening Cosmetics: Case Study In Bandung Indonesia

Objective: The objective of this research was to developing an indicator strips made from polymer polymethylmethacrylate (PMMA) to detect hydroquinone in whitening cosmetics. Methods: The strips were prepared by reagent blending method with a polymer PMMA that mixed with a chemical reagent for hydroquinone based on chemical structure: ferric chloride (FeC’3), Benedict, and ammoniacal silver nitrate. PMMA were varied with concentrations of 5%, 7.5%, and 10%. The ratios of ethyl acetate and the reagents were off 6:4; 7:3; and 8:2. Results: The results showed that the following concentration was an optimum mixture of chemical reagent-polymer and ethyl acetate FeC’3-PMMA 7.5% 7:3; benedict-PMMA 5% 6:4; and ammoniacal silver nitrate-PMMA 5% 7:3. 22 samples of whitening cream were not contained hydroquinone based on strip indicator analysis. These results are appropriate with a confirmatory test using UV spectrophotometry and thin layer chromatography. The indicator strips showed a positive result in detect the samples that had been spiked by hydroquinone but has a limitation when applied to cream dosage forms. The limit of detection (LOD) and stability of the indicator strip FeC’3-PMMA 7.5% 7:3 was 20.05 mg/I and stable up to 157 d, Benedict-PMMA 5% 6: 4 was 0177 mg/I and stable up to 123 d, and ammoniacal silver nitrate-PMMA 5% 7:3 was 0.025 mg/I and stable up to 17 4 d. The selectivity test results showed that indicator strip did not give false positive results. Conclusion: Based on the tests, the indicator strip has limitations to detect hydroquinone in whitening cream cosmetics that required further modifications to the detection process.