Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Effectiveness of the Class VI Internal Chemical Indicator Strip on Steam Sterilization of Sodium Chloride Infusion

Steam sterilization which utilizes the autoclave is an effective and fast method of sterilization. The purpose of sterilization is to provide sterile products, material and medical equipment. All goods which have been sterilized must have a sterility assurance. Sterility testing can be done based on the usage combination of mechanical, chemical and biological indicators as sterilization parameters. The purpose of this research was to find out the effectiveness of the Class VI Internal Chemical indicator strip on steam sterilization of sodium chloride infusion. This research is a laboratory experiment with procedural stages of this research includes formulation of the NaCl 0.9 % infuses, the application of class VI Internal Chemical indicator strip in the steam sterilization process and evaluation of the NaCl 0.9 % infuse which includes sterility testing.The timings of sterilization used were 5, 7, 9, 10.5, 12, 13.5 and 15 min. The results show that class VI internal chemical indicator strip shows intended color change after 12 min of sterilization. The results of the sterility test on the sodium chloride 0.9 % infuse has no microbial growth starting from sterilization time 10.5 min onwards. The class VI internal chemical indicator strip is effective with 12 min of steam sterilization at 121 °C.