Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Abnormality Analysis Of Oil Palm (Elaeis Guineensis Jacg) Somatic Embryo Using Randomly Amplified Polymorphism Dna (Rapd) And Randomly Amplified Dna Fingerprinting (Raf) Techniques

Abnormality of somatic embryo (SE) can be resulted from genetic or epigenetic changes. Thus, genetic changes can be analyzed by Random Amplified Polymorphism DNA(RAPD) and Randomly Amplified DNA Fingerprinting (RAF) techniques. The objective of this research was to study the genetic changes of SE at several growth stages and their plantlets using RAPD and RAF. Through RAPD technique the SE normal and abnormal cotyledon could be distinguished using five primers (OPE- 14,OPC-9, W-15, AP-20 and SC10-19) at clone 638, while the SE normal and abnormal cotyledon at clone 558 could be distinguished using three primers (OPE-14, W-15 and AP-20).Among these five primers pre-mentioned above, three primers, such as OPE-14, W-15 and AP-20 were differentin the SE normal and abnormal cotyledon at clone 638, at a specific band of 1750 bp. Furthermore, the RAF technique detected the changes of DNA genome at 90 – 358 bp. There were three amongstsix primers (AO-12, BB-18, W- 15) genomic DNA sequences were detected at 150 bp. Moreover, the primer AB16 could detect the sequence DNA change of SE normal and abnormal cotyledon until one base pair at 95 bp. Consequently, RAPD and RAF technique with the primers W 15 and AP 20 could distinguish normal and abnormal SE cotyledon, plantlet and normal mother plant.