Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

The Effect Of Phosphate Supply On Phosphatase Activity Of Phosphate Solubilizing Microorganisms

The phosphate solubilizing microorganisms has capable to produce extracelluler enzyme, i.e. group of phosphatase enzyme which able to mineralized of organic P to inorganic P. The objective of this experiment was to examine of phosphatase enzyme activity from phosphate solubilizing microorganism (bacteria and fungi) i.e. Pseudomonas mallei, Bacillus subtilis, Aspergilus niger and Penicillium sp. affected by phosphate supply (organic phosphate/ phytic acid and inorganic phosphate/KH2PO4) in medium.

The result of experiment showed that increasing of organic P (phytic acid ) and inorganic P (KH2PO4) affect phosphatase enzyme activity of phosphate solubilizing microorganisms and the amount of dissolve P. Furthermore, increasing of organic P substrate increase phosphatase activity; on the contrary, addition to inorganic P decrease phosphatase activity. Phosphatase activity of fungi (Aspergilus niger and Penicillium sp.) were higher than bacteria (Pseudomonas mallei, Bacillus subtilis).