Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Isolation Of Biosurfactantproducer Bacteria From Central Mud Treatment Facility (Cmtf) Pt. Bumi Siak Pusako, Riau Province

A study aims to find out and isolate biosurfactant producer bacteria originated from the Central Mud Treatment Facilities of the PT. Bumi Siak Pusako, Riau has been conducted by the end of 2008. Bacteria samples were isolated from mud and water samples. Sampling was conducted at once, in 2 sampling sites. The bacteria were then cultured using TSA, blood agar and MSM media. The presence of biosurfactant was tested using a Haemolysis Blood Agar Test (HBAT) and Index Emulsification Test (IET). Results is shown that there were 16 isolates, 8 in the mud and the other are in the water samples. Results of the IET indicate that all isolates have various level of biosurfactant production. There are 4 isolates that have 100% IET values, namely S1AI2 andS2AI2originated from water sample and S1I4 and S2I3 originated from soil sample. Using the HBAT, however, only 5 isolates from soil sample and 6 isolates from water sample shown biosurfactant production. It can be concluded that the IET method is more accurate to detect bacteria that are able to produce biosurfactant.