Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Molecular Identication And Clonal Relation Of Atypical Isolate Aeromonas Salmonicida Use Method Of Restriction Fragment Length Polymorphism ( Rflp)

Aeromonas salmonicida represent bacterium cause of furunculosis at fish resulting loss of economics in freshwater aquaculture. Counted 4 (four) A. salmonicida isolates have succeeded isolation of fish in 4 (four) region in Indonesia, but not yet been known its clonal relation. At this research to A. salmonicida isolates and ATCC atypical isolate as control have been checked its clonal relation use method of Restriction Fragment Length Polymorphism ( RFLP) by using restrictation enzyme of AluI, HaeIII, MboI and NarI. Result at examination with RFLP at phase I show the existence of difference of band of DNA at isolate 4 while 1, 2, 3 and 5 isolate showing is same pattern, goodness by using enzyme of AluI and HaeIII. Pattern of RFLP that happened is second of primary (15S and also M5) ranging from 67-421 bp where in all isolates (1, 2, 3, 4 and 5). Location difference of band at isolate 4 more which emerge band in enzyme of AluI on course 160-240 16S rDNA A. salmonicida gene compared to HaeIII endonucleus which only cutting 3 multiply in 16S rDNA A. salmonicida gene. At phase test II by using of MboI and NarI enzyme where atypical isolate of A. salmonicida (isolate of 1,2,3,4 and 5) showing result of examination do not show the existence of difference and show strong level is overall of gene sequence of 16S rDNA nucleotide where cutting a lot emerge band in enzyme of AluI on course 160-240 16S rDNA gene A. salmonicida while HaeIII endonucleus only cutting 3 multiply in 16S rDNA gene A. salmonicida