Pustaka Ilmiah Universitas Padjadjaran

Proceeding Biotech

Mutation Analysis Of Mitochondrial Dna Control Regions: A Study On Ectoderm Tissues

Mitochondrial DNA nucleotide sequence data are known and examined until the mutations level. However, comparison of mutation that occurred in the D-loop and non-D loop in the mtG on the origin of various different tissues on certain individuals who have not yet reported. D-loop is a non-coding region, containing some important sequences such as the promoter for heavy chain replication. On this research analyzed the diversity of D-loop region mtG on the various tissues that different origin on a particular individual. The aim of the research is to see if there is diversity of mtG that come from tissues that originated from the ectoderm tissue and how the mutation pattern nucleotide D-loop mtG the original came from tissue that is different. mtG analysis is done on some parts of the human body cell, such as brain and skin cells. This research begins with the individual samples in the different body tissue through an autopsy and to follow procedures / ethics applicable. Lysis cells to get the template mtDNA using QIAamp DNA Mini Kit. Then, amplified with the PCR technique using REPLI-g Mitochondrial DNA. PCR fragments of DNA D-loop region sequenced with the Sanger Method. Comparative nucleotide sequence analysis shows the diversity of mutation that occurred in the D-loop mtG on certain individuals. Nucleotide sequence data on the tissues in the region D-loop has been successfully authenticated and published in the International Nucleotide Sequence Database Collaboration (INSDC). Research determining the sequence of mtG human nucleotide of ectoderm in various tissues is involved and to contribute to frontier of knowledge. The result is expected to give benefit the development of biotechnology. Research is expected to generate future process of cooperation in the fields of biochemistry, molecular biology, bioinformatics, and forensic medicine.