Pustaka Ilmiah Universitas Padjadjaran

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Isolasi Protein Spesifik

Akumulasi logam berat di sekitar lokasi Daerah Aliran Sungai (DAS) pembuangan penambangan dapat menyebabkan keracunan pada tanaman karena tidak mampu mendetoksifikasi logam berat. Penelitian ini dirancang untuk mengetahui respon tanaman Crotalaria sp. terhadap induksi kandungan logam berat yang berbeda. Analisis laboratorium dilakukan untuk menentukan jumlah logam berat Tembaga, Timbal dan Kadmium, yang ditambahkan. Tanaman Crotalaria sp. ditanam di rumah kaca (greenhouse) dengan satu macam perlakuan logam tiap pot. Tanaman orok-orok (Crotalaria sp.) mampu hidup pada media pasir dengan kandungan logam berat Tembaga, Timbal dan Kadmium secara terpisah maupun simultan pada dosis toksik dimana akumulasi logam berat terutama terjadi pada akar. Respon tanaman terhadap kandungan logam berat yang berbeda relatif sama ditinjau pertumbuhan tanaman secara keseluruhan, tetapi pada perlakuan logam berat tanaman mengalami klorosis. Proses detoksifikasi pada tanaman Crotalaria sp. yang terinduksi logam berat dilakukan melalui mekanisme kompartemensi dengan cara menggugurkan daun terutama daun tua yang berwarna kuning. Hasil penelitian menunjukkan bahwa tanaman Crotalaria sp. yang diinduksi dengan logam berat dalam perlakuan tunggal maupun bersama-sama ternyata mensintesis sekelompok fitokelatin dengan BM  42.000 Da (termasuk HMW).

Heavy metals accumulation along the river basin area where mining disposals were dumped into could result in plants toxification as these plants were no longer able to detoxify the heavy metals. The study was designed to examine the response of Crotalaria sp. toward induction of heavy metals at various content levels. Laboratory analyses were conducted to determine the amount of heavy metals namely Cuprum, Plumbum and Cadmium, that had been added into. Crotalaria sp. was planted and nursed in a greenhouse with one treatment for each pot. The Crotalaria sp. survived in such a medium containing sand and heavy metals namely Cuprum, Plumbum and Cadmium at toxic levels either separately or simultaneously where heavy metals accumulation was profound in the rooting system. These plants response towards different heavy metal content levels was considerably similar asfar as the overall plant growth was concerned. Nevertheless, it was found that plants exposed to heavy metals suffered chlorosis. The Crotalaria sp. adopted compartmentation mechanism as its detoxification process by shedding its leaves expecially the yellowish serile leaves. The result shows that heavy metals, either separately or simultaneously induce the synthesis of a group of high-molecular weight phytochelatin of 42,000 Da.

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