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PENGGUNAAN TEKNIK PCR PADA DETEKSI GEN GTF B/C KARIES GIGI ANAK – THE PCR TECHNIC IN DETECTION OF GTF B/C GEN OF CHILDREN DENTAL CARIES
Yetty Herdiyati Nonong, Mieke Hemiawati Satari
Unpad, Presented in Bali Dance Science & Exhibition, FKG Mahasaraswati, tahun 2011
Indonesia
Yetty Herdiyati Nonong, Mieke Hemiawati Satari , Unpad, Presented in Bali Dance Science & Exhibition, FKG Mahasaraswati
caries, Gen gtf B/C, karies, PCP, PCR
Gen gtf B/C dapat dikaitkan dengan proses terjadinya karies gigi, tidak setiap bakteri yang berproduksi menyebabkan karies mengandung gen gtf B/C. Tujuan penelitian adalah untuk mendapatkan cara yang paling mudah, akurat dan mutakhir untuk mengetahui adanya gen gtf B/C penyebab karies gigi, Penelitian secara eksperimentil laboratories dilakukan terhadap 7 sampel yang diambil dan karies gigi anak. Hasil penetitian menunjukkan banyak bakteri didalam karies yang mengandung gen Gtf B/C antara lain Lactobacilus Fermentum, Lactobacilus Salivarius, Klebsiela Oxytoca, Streptococcus Mutans, Streptococcus Constelatus, Streptococcus Bovis, Streptococcus Anginosus. Kesimpulannya adalah teknik PCR memudahkan terdeteksinya gen gtf B/C yang ada pada bakteri penyebab karies,
The gtf B/C are recognized as important factor of dental caries, but not all cariogenic bacteria contained the gtf -B/C gen. The aim of the research is to determined the easy, cheap, accurate and up to date the etiology of caries, the gtf B/C gen. The research was done by laboratory experimental, samples consist 7 samples collected from children dental caries. The result showed many caries bacteria contained gtf B/C gen : Lactobacillus Fermentum, Lactobacillu Salivarius, Klebsiela Oxcytoca, Streptococcus Mutans, Streptoccocus Constelatus, Streptococcus Bovis, Streptococcus Anginosus. The conckusion is the PCR technic can detected of gtf B/C gen more easy in cariogenic bacteria.