Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Pentacyclic Triterpenes From Sarang Semut Tuber (Myrmecodia pendans) And Their Antibacterial Activity Test Against Escherichia Coli

Infectious diseases are the third leading cause of death in the world with amount of 13.3%. In Indonesia, diarrheal disease caused by infection of Escherichia coli become the first cause of death in infants in the amount of 31.4%. Myrmecodia pendans empirically has been used by the people of Papua as a drug of various diseases. In previous study, extracts of M. pendans reported have activity as antibacterial, antioxidant, anticancer, and immudolatory. This research aims to isolation and antibacterial activity test against E. coli. Isolation and purification compounds used combination of several solvent and chromatography method. The structures of the isolated compounds were elucidated by FTIR, 1H-NMR, 13C-NMR, 2D-NMR as well as by mass spectroscopic (MS) and comparing with the literature. Antibacterial activity test was done by using Kirby- Bauer methods. Two triterpenoid type pentacyclic were isolated from etyl acetate extract of M. pendans tuber. The structures were obtained through spectral analysis as oleanolic acid and pomolic acid respectively. These compounds explain the medicinal value of M. pendans tuber. The average of inhibition zone of oleanolic acid at concentration 20.000 ppm, 10.000 ppm and positive control are 10.95; 8.72; 22.65 mm. Pomolic acid also shown inhibition zone at concentration 20.000 ppm, 10.000 ppm and positive control are 12.42; 6.81 and 22.60 mm. The isolation of oleanlic acid and pomolic acid from Mymecodia genus is being reported first time and antibacterial activity test against E.coli.