Abstrak
Pengaruh Zat Pengatur Tumbuh Terhadap Pertumbuhan Gladiol (Gladiolus x gandavensis) dan Uji Resistensinya Terhadap Ekstrak Jamur Fusarium oxysporum secara In Vitro
Tia Setiawati, Titin Supriatun, Nia Rossiana
Jurusan Biologi FMIPA Unpad
Indonesia
Tia Setiawati, Titin Supriatun, Nia Rossiana, Jurusan Biologi FMIPA Unpad
Fusarium oxysporum, Gladiolus x gandavensis, IBA, in vitro, kinetin
The research to study about The Effect of Grow Regulator Substance to Growth of Gladiol (Gladious x gandavensis) and Resistance Test to Fuxorium oogupomm extract by in vitro was carried out.The reasearch conducted experimentally used a complete random design with the single factor i.e. kinetin (O ppm; 0.5 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; 3 ppm; 3.5 ppm; 4 ppm; 4.5 ppm and 5 ppm) for shoot induction and IBA (3 ppm; 3.5 ppm; 4 ppm; 4.5 ppm; 5 ppm; 5.5 ppm; 6 ppm; 6.5 ppm; 7 ppm dan 7.5 ppm) for root induction, and also Pmorium extract (0%, 10%, 20%, 30%, 40% dan 50% (v/v)) for the resistance test. The results showed that consentration of kinetin 3.5 ppm is the best for shoot induction with used_buds as an explan in solid-MS medium by adding 0.1 ppm NAA and 150 mL/ L coconut water. Consentration of IBA 5 ppm is- the best for root induction with used shoot as an explan in liquid-MS medium by adding 0.1 ppm kinetin. Consentration of Fumrium extract 30% (v/ v) could tolerance gladiol shoot.