Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Inductions Callus Various Cultivar Stevia (Stevia rebaudiana Bertoni M.) By the Addition of Variation the Concertration of 2,4-Dichloro phenoxyacetic Acid (2,4-D) on Medium Driver, Kuniyuki, Walnut (DKW)

The current sugar production decreased due to weather anomalies, and many food and beverage industries using sugar farmers. Stevia sugar has the opportunity to fill the shortfall in domestic production of sugar cane.Stevia rebaudiana Bertoni M. is one source of sweetening plant. Stevia leaves contain steviosida. Stevia is cultivated by vegetative means one of them with tissue culture.This research aims to determine the concentration of 2,4-D is most effective to induce callus on stevia leaf cultivars explant Bogor, Garut and Tawangmangu and determine the best cultivar of Stevia in response to grow callus. This research was performed using random pattern full factorial design with two factors. The first factor was the concentration of 2,4-D (D) consisting of 4 degrees of the factor are 0 ppm (d0); 1 ppm (d1); 2 ppm (d2) and 3 ppm (d3) and the second factor is a cultivar of Stevia (S) which consists of three levels of factors namely Stevia cultivar Bogor (s1), Stevia cultivar Garut (s2) and Stevia cultivar Tawangmangu (s3). The basic Media used is medium of DKW. Observed, the parameters of observation among others the wet weight of the kalus (mg), the time appears kalus (HST), the color and texture callus. The weight of wet callus analyzed using ANOVA, while time appears callus and the color and texture callus analyzed in descriptive. The result showed that the concentration of 2,4-D, cultivars and interaction between two factors influencing the weight of wet callus. Response growth callus it is best obtained on Stevia cultivars of bogor by the addition of 1 ppm 2,4-D, with time appears callus (HSI) of 7 days, a measure of callus best namely scale 16 according to clay model scales and having the weight of wet callus best of 0,734 grams. Crumb textured and thick yellow color.