Abstrak 
Intratypic Variants In Capsid Protein Gene L1 Of HPV-16 From Bandung Isolates
Edhyana Sahiratmadja, Ani Melani Maskoen, Herman Susanto
Universitas Padjadjaran, 2nd Bandung Biomolecular Medicine Conference October 5-7 2012 Molecular Oncology An Update in Clinical Application Insight on Infection Related Oncology
Bahasa Inggris
Universitas Padjadjaran, 2nd Bandung Biomolecular Medicine Conference October 5-7 2012 Molecular Oncology An Update in Clinical Application Insight on Infection Related Oncology
HPV-16, L1
Objective. Cervical cancer is associated with persistent high risk human papillomavirus (HPV) infection, with the most common infection by HPV-16 and -18. The vaccine available commercially at the moment contains major capsid protein Ll . The Ll gene of PV has several intratypic variants. Interestingly, a specific Javanese variant 6828 Tin Ll was published recently. This study aimed to explore further about the Javanese variant in Ll HPV-16 in Sundanese samples. Methods. DNA samples from biopsy of positive HPV-16 cervical carcinomas from Bandung, Indonesia (n=9) were amplified using primers specific for the Ll regions. Products were sequenced and analyzed. Results. The sequence of Ll HPV-16 showed intratypic variants among the samples. The Javanese variant 6828T in Ll region was found in 4 of 9 samples. Compared to the reference published in Genbank, our samples have deleted regions and insertions. Conclusion. The majority of the positive HPV-16 cervical cancers from Bandung, Indonesia has variants that might be associated with the ethnicity of the patients, leading to vaccine design in certain population Further bioinformatics assessment for epitopes prediction as vaccine development need to be explored in region where the HPV prevalence is high.