Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Population Build-up of Brown Planthoppers on DB1 Transgenic and Non¬Transgenic Rice Cultivars

Brown planthopper (BPH) is well-known as the major pest in rice cultivation in Indonesia. Development of BPH resistant rice cultivar is the ideal option for economic and effective management of BPH. Db1 gene (mannose-binding lectin family gene), isolated from Dioscorea batatas, is proven to be effective against some sup-sucking insects. This gene has been transferred to rice cv. Taichung-65 and currently homozygous transgenic rice line has been selected. The experiment was aimed to study obtain transgenic Obi rice lines and non¬transgenic rice lines that resistance to BPH. Genotypes used were transgenic rice cultivar of Taichung-65 with Obi insertion, non-transgenic rice cultivars i.e. Taichung-65, PTB-33, Rathu Heenati, Babawee, 1R-64, 1R-42, Ciherang, and Cisadane. Two colonies of brown planthopper i.e. colony Sukamandi (suspected a mixture of biotype 3 and 4) and colony North Sumatera (biotype 3) were used in this study. Number of BPH population was recorded from 1st to 7th observation. Results showed that cv. PTB-33 could reduce development of BPH population from 1s` to 7th observation (the last observation). From 3rd to the last observation, BPH population on transgenic rice line (Taichung-65 with DM tended to increase. Number of BPH adults on cv. PTB-33 was the least amount of BPH adults, except for colony Sukamandi. Furthermore, in this colony, the least number of adult winged BPH (Macroptera) was found on cv. 1R-42. It is concluded that population build-up of BPH is not depended on cultivar reaction.