Abstrak 
Pharmacological Activities of Plectranthus scutellarioides (L.) R.Br. Leaves Extract on Cyclooxygenase and Xanthine Oxidase Enzymes
Jutti Levita, Sri Adi Sumiwi, Tiara Indah Pratiwi, Ekky Ilham, Syafrudin Purnama Sidiq, Moelyono Moektiwardoyo
Universitas Padjadjaran, Journal of Medicinal Plants Research Vol. 10(20), pp. 261-269, 25 May, 2016 DOI: 10.5897/JMPR2016.6105 Article Number: 69FAFE858733 ISSN 1996-0875
Bahasa Inggris
Universitas Padjadjaran, Journal of Medicinal Plants Research Vol. 10(20), pp. 261-269, 25 May, 2016 DOI: 10.5897/JMPR2016.6105 Article Number: 69FAFE858733 ISSN 1996-0875
Anti-inflammatory, cyclooxygenase (COX), gout, non-steroidal anti-inflammatory drugs (NSAIDs), prostaglandin, prostaglandin H2 (PGH2), xanthine oxidase (XO).
Plectranthus scutellarioides (L.) R.Br. (family Lamiaceae) has been widely used in West Java, Indonesia, to cure various diseases. People boiled the leaves of the plant in water and consumed the tea daily until the symptoms reduced. This work was conducted to study the pharmacological activity of P. scutellarioides (L.) R.Br. extract on cyclooxygenases (COXs) and xanthine oxidase (XO) enzymes. The plant was purchased from Manoko plantation in Lembang, West Java, Indonesia. The leaves were sundried, crushed, and soaked in ethanol for 3 × 24 h, prior to be used. The extraction was continued further using ethyl acetate and water. Inhibitory activity of the extract on COXs was performed by measuring the absorbance of reduced-tetramethyl-p-phenylendiamine (TMPD) at 590 nm, which correlates to the level of PGH production, while its inhibitory on XO was measured at 290 nm. P. scutellarioides (L.) R.Br. leaves extracts (ethanolic, ethyl acetate, and water) showed inhibition on COX1 and COX-2 enzymes (40.43% for COX-1 and 97.04% for COX-2), while on XO, the water extract showed the highest inhibition (IC50 water extract = 6 µg/ml; IC50 allopurinol = 0.15 µg/ml). This plant could be further proposed as XO and nonselective COX inhibitors.