Abstrak 
OFF-LINE SPE-GC/MS FOR ANALYSIS LEAD COMPOUNDS AROMATHERAPY IN BLOOD PLASMA OF MICE OF ESSENTIAL OILS MATERIALS FROM INDONESIAN AROMATIC PLANTS
Muchtaridi Muchtaridi, Ida Musfiroh
Asian Journal of Chemistry
Inggris
Muchtaridi Muchtaridi, Ida Musfiroh, Asian Journal of Chemistry
aromatherapy, essential oils, Inhalation and GC-MS, Solid Phase Extraction
Leads compunds is one of fulfilled in standardization of Indonesian drug medicine standardized, but essential oils of material of aromatherapy standardized don’t find of essential oils material from Indonesia. The aim of this study was to evaluate the analysis method for identify lead compounds aromatherapy in blood plasma by using off–line SPE-GC/MS. Identification and quantification of active compounds in the blood plasma were carried out with GC/MS analysis after the mice experienced half an hour, one hour, and two hours inhalation. The blood plasma of three mice were collected in heparin tube and the volatile compounds were isolated and concentrated by the C-18 Solid Phase Extraction (SPE) column with methanol and bidistilled water mixture (60:40) as the solvent. The recovery analysis was increased up to 90 %. The reproducibility of extraction methods might be qualified with the indicated value of the variation coefficient less than 15 %. This method successfully detected volatile compounds in the blood plasma which the concentration was relatively low (g/mL or ppm).