Abstrak 
EFFECT OF PRE-TREATMENT AND HYDROLYSIS OF SUGARCANE BAGASSE WITH COMBINATION OF ACID-ENZYME OF A REDUCING SUGAR
Ratu Safitri, Toto Subroto dan In-In Hanidah
Panitia KIWIS 2012 yang dikoordinasi oleh Pusat Penelitian Kimia LIPI
Inggris
Ratu Safitri, Toto Subroto dan In-In Hanidah, Panitia KIWIS 2012 yang dikoordinasi oleh Pusat Penelitian Kimia LIPI
bagasse, cellulse, hemicelu, hydrolysis, Ligno cellulase, pretreatments
Sugarcane bagasse (Saccharum officinarum L.) is a readily available waste product of cane-sugar processing. The major components of bagasse are cellulose and hemicellulose. The objective of the research was to produce fermentable sugar using bagasse, involving optimization of pretreatments, sulphuric acid hydrolyses, enzym hydrolyses using respectively cellulose and hemicellulose. The experiment employed decsriptive analysies in triplicates. The result were as follows: Pretreatments of 10% (w/v) sugarcane bagasse in particle size 30 mesh required temperature 1200C; for 30 minute. The acid hydrolyses was best by using a sulphuric acid 2% (w/w) and heating steam temperatur at 1200C; for 60 minute. The enzymatic hydrolyses was best when using hemicellulose in dosage of 0,001 g/g. Followed by cellulose hydrolyses at dose of 0,083 u L/g. The enzymatic was able to hydrolyze the lignocellulose sugarcane bagasse by 65,78% with reducing sugar content 26,38 g/L.