Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Discovering COX inhibitors in Volatile Oil of Cinnamomum sintoc L.

Objective: In this work we predicted anti-inflammatory activity of volatile oil of C. sintoc L. Methods: Molecular docking was performed to predict the binding modes of eugenol, myristicin, and limonene chemical constituents of C. sintoc L. with COX enzymes, using AutoDock 4.2. COX enzymes were obtained from Protein Data Bank (PDB); COX-1 (PDB code: 2AYL) and COX-2 (PDB code: 3PGH). Flurbiprofen and celecoxib were used as standards. Further assay was carried out on lipopolysaccharide (LPS)-induced fibroblast cells reacted with 800; 400; 200; 100; 50; 25 and 12.5 ul of C. sintoc L. bark essential oils. The absorbance of the product was measured using microplate reader at 450 nm. Acetosal was used as the standard drug. Results: Eugenol and myristicin could be categorized as non-selective inhibitors of COX-2, while limonene is categorized as preferential COX-2 inhibitor. The essential oils of C. sintoc L. bark reduced PGE2 production on LPS-induced fibroblast cells. The inhibitory activity of C. sintoc L. was weaker than acetosal. Conclusion: Bioactive compounds in essential oil of C. sintoc L. bark show inhibition on PGE2 production on LPS-induced human fibroblastcells, and could be categorized as COX inhibitors