Abstrak 
THE TOLERANCE OF BACILLUS SUBTILIS AND BACILLUS PUMILUS ORIGINATED FROM BOVINE COLUSTRUM TOWARDS ACIDITY AND BILE SALT AS A PROBIOTICS CANDIDATE
Ratu Safitri, Khusnul Khotimah, Roostita Balia, Mia Miranti and Muhammad Iqbal Saputra
Departemen Biologi ITS
Inggris
Department of Biology, Faculty of Mathematic and Natural Sciences, Institut Teknologi Sepuluh Nopember – Surabaya, Indonesia Department of Biology, Faculty of Mathematic and Natural Sciences, Institut Teknologi Sepuluh Nopember – Surabaya, Indonesia, Departemen Biologi ITS
Bovine colostrum is the pre-milk fluid produced by the mammary glands of cattle after birth (Brandano et al., 2004). Colostrum also contain probiotic bacteria (Frandson, 1992). Probiotics are microorganisms that can support the balance of microflora in the digestive tract. Probiotic bacteria has the characteristics are tolerance to acidity, tolerance to bile salt, also producing antimicrobial compounds. Bacillus is a Gram positive, spores are resistant to high temperatures. Bacillus is able to grow at acidity, is able to grow at 0,2% bile salt concentrations, some Bacillus produce extracellular enzymes such as protease, lipase, amylase, and cellulase which can help the digestive system (Chou and Weimer 1999; Spinosa et al., 2000; Barbosa et al., 2005). Bacillus subtilis, Bacillus clausii, Bacillus pumilus, and Bacillus coagulans included in commercial probiotic products (Sanders, 2003). In this study we expected species of Bacillus originated from bovine colostrum that have character of acidity and bile salts tolerance so it can be used as a potentially probiotic bacteria. This study can be a basis for the used of probiotics from bovine colostrum to be developed into a commercial probiotic products.