Pustaka Ilmiah Universitas Padjadjaran

Abstrak

VOLTAMMETRIC DETERMINATION OF GENOMIC DNA USING GOLD-THIOL SELF-ASSEMBLED MONOLAYER ELECTRODE

Electrochemical methods have sensitivity, miniaturization capability, low cost and make them suitable for development of portable detection devices. The voltammetric determination of M. tuberculosis dsDNA was carried out in this study using gold electrode modified by SAM of thiols. The DPV responses of guanine of dsDNA was found at 0.2 V. The voltammogram showed a linear correlation between the current peak of guanine and the concentration of dsDNA over the concentration range 1–25 ppm (Y = 1.108X – 0.851, R2 = 0,993). This electrochemical method gives slightly different results with biophotometri but provide excellent sensitivity.