Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Factors Affecting the Acquisition of CD34+ Cells from Umbilical Cord Blood

Sufficient quantity of umbilical cord blood (UCB) affects the success of hematopoietic stem cell transplantation. This study was aimed to determine factors, which may influence the quantity of UCB. Those factors, in turn, might be controlled to optimize the acquisition of UCB. UCB samples were taken from 34 subjects, who delivered in Mother and Child Hospital Limijati, Bandung. Subjects were at term pregnancy and delivered either spontaneously or via caesarean section (C-section). Samples were phenotypically evaluated for stem cell potential with flow cytometric analysis for cell surface protein CD34+ marker. Functional characteristic of UCB hematopoietic stem cells were tested in vitro using colony forming unit (CFU) assay. Data gathered were analyzed statistically. Significant positive correlations were found between several parameters. Those correlations were between the volume of UCB and the number of total nucleated cells (TNC) (r= 0.548, p < 0.001), the concentration of CD34+ cells and the number of TNC (r =0.706, p <0.001), the hemoglobin level of the subject before delivery and the number of TNC (r= 0.387, p <0.05), and the hemoglobin level of the subject before delivery and the concentration of CD34+ cells. The number of formed CFU was positively correlated with the number of TNC (r= 0.425, p<0.05). However, it was not positively correlated with the concentration CD34+ cells. UCB with higher concentration of CD34+cells can be acquired with larger volume of UCB and higher number of TNC. The amount of formed CFU was influenced by total number of TNC but it is not affected by concentration of CD34+ cells. Further investigations are needed to determine factors, which influence the volume of UCB and the number of TNC.