Abstrak RSS

Apoptosis induced in MCF-7 human breast cancer cells by 2′,4′-dihydroxy-6-methoxy-3,5-dimethylchalcone isolated from Eugenia aquea Burm f. leaves

Apoptosis induced in MCF-7 human breast cancer cells by 2′,4′-dihydroxy-6-methoxy-3,5-dimethylchalcone isolated from Eugenia aquea Burm f. leaves
Anas Subarnas, Ajeng Diantini, Rizky Abdulah, Ade Zuhrotun, Yuni E. Hadisaputri, Irma M. Puspitasari, Chiho Yamazaki, Hiroyuki Kuwano, Hiroshi Koyama
Universitas Padjadjaran, ONCOLOGY LETTERS 9: 2303-2306, 2015 Received April 18, 2014; Accepted January 29, 2015 DOI: 10.3892/ol.2015.2981
Bahasa Inggris
Universitas Padjadjaran, ONCOLOGY LETTERS 9: 2303-2306, 2015 Received April 18, 2014; Accepted January 29, 2015 DOI: 10.3892/ol.2015.2981
, , ,

During a previous study that aimed to identify anticancer agents within primate-consumed plants, the present group identified that Eugenia aquea (E. aquea) possessed potential as a source of anticancer agents. The ethanol extract of E. aquea leaves exhibited strong inhibitory activity against the proliferation of the human breast adeno¬¨carcinoma MCF-7 cell line. The inhibition of proliferation was determined using an MTT assay. The present study was performed to isolate the active compound within the E. aquea leaves that generated the aforementioned activity, and resulted in the isolation of 2′,4′-dihydroxy-6-methoxy-3,5-di¬¨methylchalcone, which was identified through the analysis of spectroscopic data. This compound was examined for its inhibitory activity against the MCF-7 cell line using a MTT assay, and the ability of 2′,4′-dihydroxy-6-methoxy-3,5-di¬¨methylchalcone to induce apoptosis through the activation of the poly(adenosine diphosphate-ribose) polymerase (PARP) protein was also investigated. The results of the present study revealed that the isolated compound inhibited cell proliferation in a dose-dependent manner, possessed an IC50 of 74.5 14g/ml (250 14M) and promoted apoptosis via the activation of PARP. It was concluded that these results indicated a requirement for additional investigations into 2′,4′-dihydroxy-6-methoxy-3,5-dimethylchalcone in order to provide a basis for the use of this compound in the manage-ment of cancer.

Download: .Full Papers