Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Cytotoxicity of fevicordin-A from Phaleria macrocarpa (Boerl.) scheff on P388, HeLa, CasKi, TE-2, TE-8 and Prepuce’s Fibroblast cells

Cancer Is a disease caused by abnormal regulation of cells, particularly the mechanism of cell growth and differentiation. Cancer has high morbidity and mortality. The limitation of medical treatment leads to the findings of an alternative therapy using medicinal plants. Phalerla macrocarpa (Boerl.) Scheff family Thymelaeceae, is a plant which grows in tropical area, mainly In Papua Island, Indonesia, and has been used empirically to treat various health problems, amongst which are cancer, diabetes mellitus, and hypertension. Fevicordin A, Is a bloactive compound of P. macrocarpa (Boerl.) Schaff. Previous work of Kumla and his colleagues concluded that this compound has been successfully isolated from the plant and exhibited toxicity on brine shrimp larvas. The objective of this research was to investigate the cytotoxicity of fevIcordin A on P388, HeLa, CasKi, TE-2 and TE-8 cancer coils, while Its effect on normal calls was performed on fibroblast cells isolated from human prepuce skin. The method used was MT assay by using 3-(4,5-dimethytthlazol-2-y1)-2,5- dlphenyttetrazollum bromide reagent. The results of the work indicated that IC50 values of fevicorclin-A on the growth of the cancer cells were 0.01 pg/mL (P388), 1.16 pg/mL (HeLa), 12 pg/mL (CasKi), 40 pg/mL (TE-2), and 14.6 pg/mL (TE-8), whereas the IC.,30 on the growth of fibroblast cells was 24.51 pg/mL These results suggest that fevicordin A may have potential as a cytotoxic agent against leukemia and cervix cancer.