Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Aktivitas Antijamur Ekstrak Daun Lidah Buaya Terhadap Candida Albicans Yang Diisolasi Dari Pasien AIDS (The Antifungal Activity Of Extract Of Aloe Barbadensis Mill. Leaves Against Candida Albicans Isolated From AIDS Patient)

Oral candidiasis is the most common opportunistic infection in patients with AIDS. Candida albicans is by far the most common species causing oral candidiasis. Many of the difficulties faced in treating oral candidiasis in AIDS patient have been reported, such as resistance to antifungal agents and clinical adverse effects. Aloe barbadensis Mill. is one of the most widely used as herbal remedies. It has been reported that Aloe extracts have vitro antibacterial, antifungal, and antiviral activities. Up to now, there are only a few reports in the literature on the an antifungal activity of Aloe extract against C. albicans. The aims of this research were to evaluate antifungal activity and to determine minimum inhibitory concentration (MIC) of an extract of Aloe barbadensis Mill. leaves against C. albicans isolated from AIDS patients. This study was an experimental laboratory. Candida albicans isolated from the AIDS patient with acute pseudomembranous candidiasis, then identified based on the colony morphology, Gram painting. and sugar fermentation test. The minimum inhibitory concentrations of the Aloe extracts against C. albicans were determined by serial dilution technique. To compare the antifungal activities, ketokonazol was used as antifungal standard. The results showed that extract of Aloe barbadensis Mill. leaves has antifungal activity against C. albicans with MIC ranging from 4.5% to 5%. The comparison test of Aloe extracts and ketoconazole as an antifungal showed that to give the same antifungal activity against C. albicans was needed 1 ppm ethanol extract of Aloe comparable with 4,8059×10-3 ppm ketoconazole.