Pustaka Ilmiah Universitas Padjadjaran

Abstrak

The Relationships Between Sorbitol Dehydrogenase (SDH) Level and Diabetic Rethinopathy in Diabetes Melitus Type-2 Patients

Background: The conversion of sorbitol accumulation to fructose by SDH triggers the osmotic damage in retinal endothelial cells and pericyte through the activation of advanced glycation end products (AGEs), oxidative-nitrosative stress, Protein Kinase C (PKC) pathway activation, inflammation, and the imbalance of growth factor. The osmotic damage eventually leads to DR (Diabetic Rethinopathy). Methods: This is a case-control study. DM type 2 patients who had the direct ophthalmoscopy and fundus imaging in dr. Mohammad Hoesin Central General Hospital opthalmology outpatient in Palembang was selected as a subject in this study.Patient’s blood sample was collected from a mediancubital vein for 3 mL and stored in ethylene diaminetetraacetic acid (EDTA) coated tube for SDH assay by ELISA. Results: The average level of SDH in DR subjects was 13,3 ±7,8 ng/mLand the average level of SDH in Non DR was 10,7±2,3 ng/mL, p=0,044.There was no significant difference in subjects with DR and subjects in control group in level of SDH by chi square-test, which the level of SDH was devided into two groups, higher than 11,18 ng/mL and lower than 11,18 ng/mL by ROC curve. Conclusion: There was significant difference in subjects with DR and subjects in control group in level of SDH. The average of SDH level in DR subjects were more higher than Non DR subjects.