Abstrak 
Measurement of Uric Acid Level In Vivo by Reverse Iontophoresis
Nasrul Wathoni, Alya Nur Hasanah, Anandhita Utami
Universitas Padjadjaran, International Journal of Emerging Technologies in Computational and Applied Sciences, 4 (3), March-May, 2013,pp.319-324, ISSN (Print): 2279-0047, ISSN (Online): 2279-0055, www.iasir.net
Bahasa Inggris
Universitas Padjadjaran, International Journal of Emerging Technologies in Computational and Applied Sciences, 4 (3), March-May, 2013,pp.319-324, ISSN (Print): 2279-0047, ISSN (Online): 2279-0055, www.iasir.net
in vivo, invasive, rats, reverse iontophoresis, uric acid
The routine method of the blood sampling for measuring uric acid level is achieved by using a syringe which is an invasive method. Reverse iontophoresis is an alternative non-invasive method for sampling of uric acid in the blood. The aim of the present work was to determine the effectiveness of reverse iontophoresis method on measurement of uric acid level in rat. The research was conducted by using an in vivo modified diffusion cell and wistar strain male rats. The reverse iontophoresis method was used 0,5 mA/cm2 current density with a modified iontophoresis device. The average uric acid levels after diffusion testing for 6 hours was 1.73 ppm, while in the blood were 63.8 ppm. The coefficient correlation between the levels of uric acid in the blood and the levels of uric acid in the receptor fluid was 0.5215. In the previous study, the in vitro study indicated that uric acid had been successfully extracted through the abdominal skin of rat to a collection solution of modified diffusion cell as much as 7.35 ppm. In conclusion, in vitro and in vivo studies showed the possibility to describe the levels of uric acid from interstitial fluid with reverse iotophoresis method.