Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Effect Of Isolate Quercetin-3-o-glucoside (Q3G) From Neem Leaf (Azadirachta Indica A. Juss) In Diabetic Rats Model (Rattus Rattus Novergicus)

Diabetes mellitus is a chronic disease marked in increased concentration of blood glucose. For decreasing blood glucose, hypoglycaemic drugs could be used. Quercetin-3-O-Glucoside (Q3G) was isolated from neem leaves (Azadirachta indica A. Juss) acts as antidiabetic. The aim of this research wants to find out the effect of isolate Q3G for decreasing blood glucose of diabetic rat models. We randomized 24 white-male Wistar rats, weight 150-250 gr, into 4 group, negative control, positive control and two conduction group, was given Q3G 1 mg/kgBW, and Q3G 2 mg/ kgBW. The rats had treated after one-week adaptation at Animal Laboratory. After conduction, all rats were given glucose 6,25 mg/kgBW orally. Blood glucose level were taken from rat’s tail and measured by glucometer at before the conduction, 1, 2 and 4 hour after the glucose. Repeated One Way ANOVA were used to analyse the data with p = 0,05. Rat’s Blood Glucose level decreased 143,11 (-34,72-320,95) mg/ dl and 71,22 (-106,61-249,06) mg/dl after had given orally Q3G 1 mg/kg and Q3G 2 mg/kg vs. aquades. The glucose level more clinical significant while compared with acarbose, 47,24 (-141,11-235,59) and -24,65 (-213-163,7) mg/dl, p>0,05.The statistics results showed decreasing of blood glucose level by Q3G 1 mg/kgBW and 2 mg/kgBW is not significant difference with group which given aquades and acarbose.