Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Ekspresi Koreseptor Human Immunodeficiency Virus CCR5 Dan CXCR4 Pada Subset Sel Limfosit T Serta Monosit (Human Immunodeficiency Virus Coreceptor CCR5 and CXCR4 Expression on Lymphocyte T Subset and Monocyte)

Reseptor kemokin CCR5 dan CXCR4 terletak di permukaan sel limfosit dan memegang peranan penting dalam infeksi HIV serta patogenesisnya. Penunjukan kedua koreseptor di sel yang berbeda ini akan menentukan perjatanan penyakit infeksi HIV Tujuan penelitian ini adalah untuk mengetahui sebaran subset sel limfosit T dan monosit serta melihar penunjukan koreseptor CCR5 dan CXCR4 di set limfosit T orang sehat. Kajian ini merupakan penelitian pendahuluan untuk melihat sebaran penunjukan koreseptor CCR5 dan CXCR4 orang sehat dengan bahan pemeriksaan berupa sel darah tepi intl runggal/perlpheral blood mononuclear cells (PBMC). Pengenalian subset sel limfosit T clan monosit serta penunjukan koreseptor CCR.5 dan XCR4 dilakukan dengan metode ukuran aliran set (flowsitometri). Sel limfosit T ingatan (memory) merupakan subset set timfosit T terbesar (rerata 66,2%) sementara set timfosit pengatur yang dikenali dengan runjukan tinggi CD25+ hanya ditemukan antara 2,0-5,3%. Koreseptor CXCR4 ditemukan berpetunjuk di lebih banyak sel daripada koresepror CCR5 di semua subset set limfosit T clan monosit. Sebagian kecit (2,85%) monosit yang menunjukkan kedua koreseptor, sementara sebagian besar sel limfosit menunjukkan balk koreseptor CCR5 clan CXCR4. Sebaran reseptor CCR5 clan GXCR4 hampir sama di set timfosit I CD4 maupun di monosit. Di sel timfosit T pengatur diremukan runjukan CXCR4 yang jauh lebih rendah (18,18%) dibandingkan dengan set lain, tetapi kekuatan fluoresen set yang menunjukkan kedua koreseptor (CCR5 53,35 dan CXCR4 92,33) sangat tinggi. Sebaran runjukan koreseptor HIV CCR5 dan CXCR4 yang berbeda di subset set limfosit T dan monosit baik jumtah set yang menunjukkan kedua koreseptor dan kekuatan tluoresen di senap setnya.

Chemokine receptors CGR5 and GXGR4 which lied on lymphocyte cell surface play important role in HIV infection and pathogenesis. The expression of these chemokine receptors will affect progressively the disease. The objectives of the study are to find the distribution of lymphocyte T cell subset and monocyte among the peripheral blood mononuclear cells and to know the determination of CGRS and CXCR4 co receptors expression on T lymphocyte cells subset and monocyte. This study is a preliminary study to explore the distribution of co receptors CCRS and CXCR4 expression in healthy people. The sample taken is peripheral blood mononuclear cells (PBMC) from healthy subjects. The identification of T lymphocyte cells subsets and monocyte, and the expression of CCR5 and CXCR4 co receptors were determined using flowcytometry. The memory T cell (CD4+CD45RO) is found to he the largest proportion among T lymphocyte cell (66.2%), whereas the other T lymphocyte cell subset, regulatory T cell, which identified by CD25+ high expression was found between 2.0-5.3% from the whole T lymphocyte cell. The proportion of CXCR4 co receptors was found higher compare to CCR5 co receptors on all Tlymphocyte subsets and monocyte. Only small proportion of monocyte expresses both co receptors (2.85%), but most of the T lymphocyte cell expressed both CCRS and CXCR4. The expression of the CXCR4 on regulatory T cell (18.18%) is the lowest compared to other cells, but the fluorescence intensity of both co receptors was very high (CCR5 53.53 and CXCR4 49.33). The different distribution of CCR5 and CXCR4 co receptors among T lymphocyte cell subsets and monocyte will influence the vulnerability and the pathogenicity of HIV infection.