Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Relationship between Temperature and Humidity on Sterility of Reusable Instruments in Hospital’s CSSD

One of the activities for healthcare associated infections control (HAIs) is sterilization of reusable materials and instruments before being distributed is being stored in a storage room. In this storage room, possible contamination can occur. The purpose of this study is to analyze the relationship between the temperature and humidity of the storage space and the sterility of reusable instruments in hospital’s CSSD. A sequential explanatory with mixed methods was done. Quantitative research was conducted on the measurement of temperature and humidity of storage space by using a thermo-hygrometer with ten sampling locations, examination of the total number of germs room with settling plate method, sterility testing, identification of bacteria by Gram staining and biochemical tests. The bivariate data was analyzed with Pearson correlation and Eta association. The multivariate analysis used was linear and logistic regressions. The qualitative study was carried out with interviews and observations, which were analyzed by content analysis. The quantitative results showed that there was a significant relationship between temperature and humidity to sterility of the storage space. In the other hand, there was no relationship between the temperature, humidity and the sterility of storage room on reusable instrument. The bacterias isolated from storage space were Escherichia sp., Klebsiella sp., Salmonella sp., Staphylococcus sp., and Streptococcus sp. Factors on sterile supply cycle process that could affect to this situation is handling of these factors were not standardized, such as decontamination, packaging, storage and monitoring of the final product processes. There may be other risk factors associated with reduced security of reusable instruments used in hospital, such as hospital staff personnel hygiene, washing water quality, and room air filtration. These could be the possible risk of sterility assurance of reusable instruments in hospital.