Pustaka Ilmiah Universitas Padjadjaran

Abstrak

A New Approach Of Ofloxacin Analysis Method In Human Blood Plasma Using Solid-phase Extraction – Highperformance Liquid Chromatography-ultra Violet

Objective: The aim of this research was to develop a method for analyzing ofloxacin (OFX) assay in human plasma using solid-phase extraction (SPE)- high-performance liquid chromatography (HPLC)/ultra violet (UV) detector. In this work, SPE was employed in preparing for the analysis of OFX using HPLC-UV detector. Materials and Methods: Hydrophilic and lipophilic balance cartridge (100 mg, particle size 10 µm) of SPE was used in preparing a sample to determine further method of analysis using HPLC with phosphate buffer 0.025 M (pH 2.5) and acetonitrile (85.5:14.5) as mobile phase and a flow rate of 1.2 m l/min. UV detector was adjusted at 295 nm using internal standard ciprofloxacin. Results: Calibration curve was linear over the range of 0.1-6 µg/ml with correlation coefficient (r) = 0.9998-0.9999. The resolution was (Rs) >1.5, and repeatability (% CV) <10%. Based on peak area and the peak height ratio of chromatogram, limit of detection and limit of quantification were 0.023 µg/ml and 0.076 µg/ml, respectively, and recovery of spiked OFX in human plasma was 94.32-100.45%. Conclusion: Based on the results of analysis, the analysis method was concluded as sensitive and valid for analysis of OFX in human plasma.