Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Cytotoxic Activity From Ethanol Extract And Fractions Sablo (Acalypha Wilkesiana) Leaves On Hela Servical Cancer Cells

Cancer is still one of the main health problems in the world. Cervical cancer is a leading cause of cancer deaths in women worldwide. In parts of the developing world, cervical cancer is the major cause of death in women of reproductive age. Conventional therapies to treat cancer are still unsatisfied. Alternative therapy especially from natural materials is needed. One of the natural materials that has the potential to be developed as cancer drugs is sablo (Acalypha wilkesiana) leaf. This study was conducted to determine the cytotoxicity of extracts and fractions of sablo leaves against HeLa cervical cancer cells with MTT (3-[4,5dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) Assay. The results showed that the ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction of sablo leaves had cytotoxicity against HeLa cervical cancer cells with IC 50 value of 98.91 µg/mL, 88.85 µg/mL, 79.84 µg/mL and 102.47 µg/mL, respectively. The cytotoxicity on HaCaT normal cells showed that the ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction provided a cytotoxicity effect with IC 50 values of 217 µg/mL, 115 µg/mL, 425 µg/mL, and 617 µg/mL, respectively. The results of this study indicate that sablo leaves of ethyl acetate fraction was quite effective to inhibit the proliferation of HeLa cervical cancer cells and might have potential for cancer therapy.