Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Activity of Cytotoxic Flavanoids against a P-388 Murine Leukemia Cell Line from the Stem Bark of Aglaia elliptica (Meliaceae) (Aktivitas Sitotoksik Senyawa Flavanoid Terhadap Sel Murine Leukemia P-388 dari Kulit Batang Aglaia elliptica (Meliaceae))

Dua campuran senyawa flavanoid (1 dan 2), suatu campuran dari katekin (1a) dan epikatekin (1b) serta campuran dari gallokatekin (2a) dan epigallokatekin (2b) telah diisolasi dari kulit batang Aglaia elliptica. Struktur kimia senyawa tersebut diidentifikasi berdasarkan data spektroskopi, meliputi UV, IR, NMR (1H,13C, DEPT 135°, HMQC,HMBC,1H-1H COSY) dan MS, serta tambahan dengan perbandingan data spektra yang diperoleh sebelumnya. Semua senyawa dievaluasi aktivitas sitotoksiknya terhadap sel murin leukemia P-388. Senyawa 2 menunjukkan aktivitas sitotoksik terhadap sel murin leukemia P-388 dengan nilai IC 7,79 µg/mL, sedangkan senyawa 1 tidak memberikan aktivitas sitotoksik (IC 50 lebih besar dari 100 ug/mL). 50

Two mixtures of flavanoid compounds (1 and 2), a mixture of catechin (1a) and epicatechin (1b), and a mixture of gallocatechin (2a) and epigallocatechin (2b), were isolated from the active fraction of the stem bark of Aglaia elliptica methanol extract. The chemical structure of the compounds was identified with spectroscopic data, including UV, IR, NMR ( 1H,13C, DEPT 135°, HMQC, HMBC, 1H-1H COSY), and MS, and additionally compared with previously reported spectral data. All compounds were evaluated for their cytotoxic effects against P-388 murine leukemia cells. Compound 2 showed cytotoxicity against the P-388 murine leukemia cell, with an IC value of 7.79 µg/mL, but compound 1 was found not to be active (more than 100 µg/mL).