Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Antibacterial Activity Of Papuan Ant-nest (Myrmecodia Pendans L.M. Perry) Ethanol Extract Against Z

Background: Shigella dysenteriae continues to be a major health problem in Indonesia, which usually leads to death, due to diarrhoea and dysentery, predominantly in children below the age of 5. Bacterial invasion of the colonic epithelium leads to severe inflammation together with bacterial dissemination generates abscesses and ulcerations. Myrmecodia pendans, also locally known by indigenous Papuans as ant-nest is native to Southeast Asia. This tropical plant has proven to be rich in bioactive constituents and highly valued as an alternative choice for cancer/tumor treatments and an efficacious herbal drug to prevent and cure diarrhea. Objective: This research aimed to determine antibacterial activity of Ant-nest extract against S. dysenteriae and to determine its minimum inhibitory concentration (MIC) – minimum bactericidal concentration (MBC) range of concentration. Methods: Ant-nest dried plant were obtained from Wamena, Papua. The extract was obtained by using maceration method with 70% ethanol as solvent. Antibacteria activity test were then performed by using perforated agar method with various extract concentration (10, 20, 40, and 60% (g/mL)). MIC-MBC concentration determinated by using microdilution method. Results: From 500.42 g dried plant we can obtain 77.47 g dry extract (15.48% rendement). Phytochemical screening result showed that the ethanol extracts of ant-nest contains metabolites such as alkaloids, flavonoids, tanins, saponins, and steroids/terpenoids. Largest inhibition zone was shown by 60% extract concentration with 1.74 ± 0.021 cm diameter. MIC – MBC concentrations lays in range of 14 – 16 % (w/v). Conclusion: The results of the present investigation suggest that the extracts of the studied plants can be used as potential leads to discover new drugs to control some S. dysenteriae infections.