Pustaka Ilmiah Universitas Padjadjaran

Abstrak

The Potential Activity Of Ethanolic Extract From Antnest Plant (Myrmecodia Pendens Merr.And L.M Perry) Against Hyaluronic Acid And Essential Oils Of Plant-resistant Staphylococcus Aureus

Aim: The objective of this study was to evaluate the antibacterial activity of ant-nest plant ethanol extract against Staphylococcus aureus clinical isolates and determined its minimum inhibitory concentration (MIC). Materials and Methods: The simplisia of ant-nest plant was extracted using a maceration method. The phytochemical screening was conducted using standard methods. The antibacterial activity was done by the agar diffusion method using chlorhexidine gluconate as comparative drug. The MIC test was done by a macrodilution method and following by subculturing the overnight result onto the surface of agar media. Results and Discussion: Phytochemical screening revealed the presence of polyphenols, flavonoids, tannins, and saponins. Antibacterial activity of ant-nest extract exhibited the anticaries activity against S. aureus clinical isolates in terms of inhibitory zone formation. The chlorhexidine gluconate in a concentration of 0.025 %w/v had given inhibition effect to all S. aureus isolates. The MIC value of ant-nest extract was ranging at concentrations of 0.825–1.75% w/v. Conclusion: The ethanolic extract of ant-nest plant potent for S. aureus that had been resistant against hyaluronic acid and essential oils of plant.