Abstrak 
Studied of CD14 and CD15 Gene Expressions for Myeloid Derived Suppressor Cell Profile at the RNA Level as a Predictor for Progressivity in Nasopharyngeal Carcinoma
Dewl YA, Shahib MN, Boesoirie T, Achmad D
Universitas Padjadjaran, Avens Publishing Group Journal Of Surgery, February 2016 Vol: 4, lssue:1, ISSN: 2332-4139
Bahasa Inggris
Universitas Padjadjaran, Avens Publishing Group Journal Of Surgery, February 2016 Vol: 4, lssue:1, ISSN: 2332-4139
2-Ct Methods, CD14, CD15, Myeloid derived suppressor cell, Nasopharyngeal carcinoma, Predictor of progression, qRT-PCR
Background: Nasophoryrigeal carcinoma (NPC) is o disease in which malignant cells formed in the tissue of the nasopharynm which is a frequent cancer in Indonesia. Estimated of NPC in Indonesia at 6.2/100.000 or about 12.000 new cases per year. Developments of tumors are influenced by many factors, generally an interaction between genetic, environmental and immune system. There is a role of myeloid derived suppressor cells in the process of immune suppression. Objective: The aim of this study is to clarify the profile of MDSC encoded by CD14 and CD15 genes expression to find the predictor for progressivity of nasopharyngeal carcinoma. Peripheral blood specimen and biopsy from primary tumor were collected from 16 nasopharyngeol carcinoma patients. The samples collected underwent qRT-PCR. Data were analyzed by 2-Ct methods and statistical analysis. R.sults: There were op regulation of CD14 and CD15 m-RNA level in blood at advanced stage (52.42 and 38.03) using 2-b.Ct methods. The expression of CD14 and CD15 genes was strongly correlated with clinical stage. Conclusion: MDSC can be used as a predictor of progression In NPC.