Pustaka Ilmiah Universitas Padjadjaran

Abstrak

The Role Of Myeloid Derived Suppressor Cells And CXCR4 Genes Expression For Nasopharyngeal Carcinoma Progression

Nasopharyngeal carcinoma (NPC) is radiosensitive, but prognosis remains poor with a 5-year survival around 50% due to secondary spread of tumor cells. Tumors growth is influenced by many factors, generally an interaction between genetic and environmental factors, particularly the microenvironment tumor. There is the role of myeloid derived suppressor cells (MDSC) in the process of tumors growth. MDSC are immature myeloid cells produced by bone marrow precursor cells that are increased in a variety of disease. Most significantly, MDSC are increased in cancer patients and significantly contribute to the immunosuppression. MDSC are then recruited to the tumor by such chemotactic factors as tumor derived CXCL12 and stem cell factor that bind to and active their respective receptors CXCR4 on MDSC. The main role of MDSC appeared to be due to immunosuppression of anti-tumor effectors and had significant effects of tumor progression. This study identified specific markers that can be used to identify MDSC and the relation with CXCR4 for NPC progressivity prediction. Peripheral blood specimen and biopsy from primary tumor were collected from 16 nasopharXneal carcinoma patients. The samples collected underwent qRT-PCR. Data were analyzed by 2 methods and statistical analysis. CD14, CD15, and CXCR4 genes are expressed in peripheral blood and primary nasopharyngeal carcinoma. We found that patients with advanced stage had elevated number of circulating CD14, CD15, and CXCR4 although the increase of CXCR4 did not reach the significant level. MDSC and CXCR4 correlated significantly with T and N classification and clinical stage also. We concluded that expression of MDSC and CXCR4 play an important role in tumor progression and invasion in NPC.