Pustaka Ilmiah Universitas Padjadjaran

Abstrak

Acute Iron Dextran Injection Increases Liver Weight and Reduces Glycerol Kinase Expression in Liver

Introduction: Iron is essential and needed in a very small amount. When iron exceeds normal need, metabolic alteration occurs, causing hepatosteatosis. The mechanism of iron causing hepatosteatosis is yet known. Glycerol kinase, enzyme responsible in triglyceride synthesis initiation, is assumed to have role in pathomechanism of hepatosteatosis. Objective: To investigate the expression of glycerol kinase in acute iron overload condition. Method: This study was conducted in Animal Laboratory Faculty of Medicine and Central Laboratory Universitas Padjadjaran from May to June 2017. Three groups of mice were divided by dose of iron dextran injection (0, 0.1, 0.3mg/day/mice). After 19 days, Mice were terminated, liver weight was measured and glycerol kinase expression in liver was determined by semiqualitative PCR.Quantification of PCR result was calculated by ImageJ software. Result : There were significant change in liver weight of the mice appropriate with dose of iron injection. The expression of glycerol kinase were decreasedfound, but statistically insignificant. Conclusion: Acute iron dextran injection increases liver weight and reduces glycerol kinase expression in mice liver.