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A Novel Of Bezafibrate Analysis Methods In Urine (In Vitro) Using Solid Phase Extraction– High-Performance Liquid Chromatography-UV Detector
Iyan Sopyan, Widiastuty Irawati, Wiwiek Indriyati
Universitas Padjadjaran, International Journal of Applied Pharmceutics Vol 10, Issue 1, 2018, 94-97, ISSN- 0975-7058 , DOI: https://dx.doi.org/10.22159/ijap.2018v10i1.22769
Bahasa Inggris
Universitas Padjadjaran, International Journal of Applied Pharmceutics Vol 10, Issue 1, 2018, 94-97, ISSN- 0975-7058 , DOI: https://dx.doi.org/10.22159/ijap.2018v10i1.22769
Bezafibrate, HPLC, SPE, Urine
Objective: Bezafibrate is the second generation of fibrate groups used as the drug of choice in the treatment of hyperlipidemia. The purpose of this study is to obtained a validated method for analyzing bezafibrate in urine using solid phase extraction (SPE)-High performance liquid chromatography (HPLC). Methods: Solid phase extraction (SPE) using hydrophilic-lipophilic balance (HLB) cartridge was performed for bezafibrate extraction from urine, afterward, a validation of analysis method using high-performance liquid chromatography (HPLC)-(UV) detection was conducted to parameters, including: selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). Results: Recovery extraction using SPE resulted %recovery 85-110%. The analysis was performed by high-performance liquid chromatography using reversed phase, C18 octadecylsilane (ODS) columns 250 x 26 mm, particle size 10 µl, with the composition of 0.01 M acetate buffer with pH 3.55: with percent composition (45:55) and 0.8 ml/minute on 230 nm UV detection. Validation includes selectivity, linearity, accuracy, precision LOD, and LOQ have fulfilled requirement value. Conclusion: The result of recovery extraction using SPE and validation of method exhibited the values that fulfilled the requirements and can be used for analysis bezafibrate in the urine.